The most suitable agent for extracting cannabis plant samples was evaluated to be a mixture of acetonitrile and water in a ratio 1:1. For all target analytes, the %-Bias at the lower and upper calibration range varied from - 1.3 to 10.3% and from - 3.9 to 8.6%, respectively. Results The optimized method is able to separate cannabidivarin, cannabidiolic acid, cannabigerolic acid, cannabigerol, cannabidiol, cannabinol, 9-tetrahydrocannabinol, and tetrahydrocannabinolic acid within 10 min. ![]() Methods We report the successful development and validation of an accurate and broadly applicable reversed-phase high-performance liquid chromatography (RP-HPLC) method coupled to an ultra violet (UV) detector including an optimized extraction procedure for the separation and quantification of eight different cannabinoids. In general, gas chromatography (GC) is the method of choice for the quantification of cannabinoids whereas this method is time consuming and the detection of acidic precursor is not feasible without derivatization. Therefore, the reliable and fast quantification of cannabinoids in hemp samples is essential for the control of product from Cannabis sativa, L. ![]() Abstract : Background Currently, an increasing demand of cannabis-derived products for recreational and medical use is observed.
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